If the sample volume and concentration meet specifications, libraries are tested with the Agilent bioanalyzer and qPCR. Novogene has strict quality control standards; Only qualified samples can be used for NGS. Quality tests are performed prior to downstream bioinformatics analysis. We validate the quality of the sample, library and data. To send DNA samples, you have the following options: High-quality total RNA as starting material for an mRNA sequencing project is crucial for the successful extraction of bioinformatics information from sequencing data. Novogene performs strict quality controls to help you select high-quality total RNA samples for sequencing and avoid potential downstream issues. In addition, in this webinar we will also share our extensive experience as a guide for preparing samples from tissues. · Choosing the right RNA sequencing strategy and approach · Sample requirements for RNA sequencing · RNA-seq Data Considerations and Analysis This webinar will also provide you with a detailed understanding of our specific requirements and recommendations for sample storage and transportation to avoid common mistakes that would otherwise affect your valuable samples and project schedules. The webinar consists of a 30-minute presentation followed by a question and answer session. This paper shows the need for Novogene samples of DNA, RNA and libraries for different types of sequencing. It is important that the proper instructions are followed to ensure the success of your project. If your samples do not meet our standards, please contact us for evaluation. To ensure successful preparation and sequencing of the library, all samples received by Novogene are first subjected to quality control.

The quality of DNA samples is measured by agarose gel electrophoresis and Qubit 2.0. The quality of RNA samples is assessed using NanoDrop, agarose gel electrophoresis and the Agilent 2100 bioanalyzer. In this webinar, you will learn more about the specifics of the sample receipt process and Novogene`s guidelines. We will discuss how the transition to our new Customer Service System (CSS) will improve your sample submission experience and give you more control to ensure your samples can be processed as soon as they arrive. Novogene`s mRNA sequencing, based on state-of-the-art Illumina NovaSeq platforms, is widely used to profile the mRNA snapshot or even the entire transcriptome at a given time. It provides comprehensive solutions to analyze gene expression and differential gene expression between sample groups, as well as to identify gene structure variations, such as alternative splicing, gene fusion events, etc. Sample quantities above minimum requirements can improve library success rate When preparing the NGS library, the first samples are fragmented/sheared, trimmed, ligated with modified oligos, the size selected and amplified. Accurate qualification and quantification of the library is critical to successful sequencing. The quality control process for the Novogene library consists of four steps: Novogene does not return any library created by us.

If you prepared the libraries yourself, we can return them to you. Please refer to the question above regarding information on return shipping costs. Before sending samples, you will receive a Sample Information Form (SIF) to complete. After completing the form, please return an electronic copy and attach a printed copy to your sample package. For sample requests, shipping address and shipping instructions, please contact europe@novogene.com (Europe), amea.pc@novogene.com (Hong Kong, Macau, Taiwan, Singapore, Southeast Asia, Middle East, Japan, South Korea, Australia, New Zealand, India, Africa), inquiry_us@novogene.com (North and South America) or contact your local account manager directly. Note: In America, Novogene does not accept USPS as a shipping service provider. Please choose other carriers like FedEx, UPS, DHL, etc.) Novogene is focused on customer success. Customer success begins with project design, efficient project execution, data analysis, and customer support to ensure that the data and reports generated meet their needs.

To achieve all of these goals, we have teams for each project working with the client to ensure that we understand their needs and meet them to the client`s satisfaction. At Novogene, we view a successful project as one in which we have effectively and efficiently generated data ready for publication in a timely manner, giving our clients what they need to succeed in research. RNA-Seq (RNA sequencing or transcriptome sequencing) studies the quantity (quantification) and sequences (isoform identification) of RNA using various sequencing technologies. In this webinar, our technical expert will walk you through the basics of RNA sequencing and introduce you to some bioinformatics sequencing and analysis services used for different types of RNA, including mRNA sequencing, ncRNA sequencing, whole transcriptome sequencing sequencing. A considerable staff of bioinformaticians to analyze your data quickly and efficientlyAdvanced Titan Krios 300 kV cryoelectron microscope analysis service is available. We cordially invite you to our next free webinar! By carefully and carefully monitoring the quality of sequenced DNA and RNA, we can increase the quality of the results we share with our customers. Measures concentration to ensure the best amount of material is used for optimal results RNA is a single-stranded nucleic acid polymer of the four nucleotides A, C, G and U, which is connected by a backbone of alternating phosphate and rib residues and converts information from DNA into proteins or plays a direct role in cells. such as rRNA, tRNA, etc. Of all types of RNA in cells, messenger RNA (mRNA) is a molecule that transports DNA codes in the nucleus to protein synthesis sites in the cytoplasm (ribosomes). Provides library sizing and quality assessment Quality control of raw data is an important measure in determining the quality of libraries and whether sequencing has succeeded or failed.

Novogene will rigorously analyze sequencing data to ensure accuracy and reliability, including quality, error rate, Q20, Q30, adapter contamination rate, etc. Low-quality raw reads containing adapter sequences or containing >10% of basic “N” calls will be removed to keep playbacks clean. Generating useful and actionable information from the NGS is often difficult and intimidating. Novogene`s expert staff helps our customers solve this problem. Our experienced bioinformaticians help our clients generate the data they need to advance their research. Our scientists combine bioinformaticians and automatic analysis systems. Our computer analysis far exceeds that of all other service providers. In addition to analyzing our clients` data and providing comprehensive analysis, we provide comprehensive support to improve the efficiency of our clients` research and work closely with them to generate results ready for publication. Our bioinformaticians are highly experienced and “specialists” in the types of analyses they perform. The specialization allows them to gain experience and knowledge in this area of analysis, refine their skills and provide the best possible results and support. With Dr. Li`s exceptional bioinformatics capabilities and application orientation, we have cutting-edge analytical expertise that enables us to deliver publish-ready results to our clients.

Novogene is headquartered in Beijing. As the global leader in NGS, we also have offices in Hong Kong, Singapore, the United States and the United Kingdom. Unrivalled bioinformatics expertise for each project. We offer DNA/RNA extraction services for tissues or body fluids. If you require DNA/RNA extractions, please contact your Novogene representative to discuss your specific needs. However, the preferred method is to send extracted genetic material. *Processing time starts from project confirmation to data release. Please contact your Novogene representative before sending your cheque.

Send your check directly to our office at the following address: Americas Mailing Address: Novogene Corporation Inc. 8801 Folsom Blvd #290, Sacramento, CA 95826 Singapore and Southeast Asia Mailing Address: NovogeneAIT Genomics Singapore Pte Ltd. 25 Pandan Crescent, #05-15 TIC Tech Centre, Singapore 128477 Hong Kong, Macau, Taiwan, Middle East, Japan, South Korea, Australia, postal address for New Zealand, India and Africa: Novogene (HK) Company Limited Level 54, Hopewell center, 183 Queen`s road east, HONG KONG.